Radioimmunoassay of progesterone in unextracted serum.
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of triiodothyronine in unextracted human serum.
Serum triiodothyronine (T-3) concentrations have been estimated by radioimmunoassay using unextracted serum. The serum T-3 concentrations have been shown to be similar in two separate European populations (0.76-1.67 ng/ml). Raised T-3 values have been observed in all subjects with hyperthyroidism. Low values are seen in hypothyroidism although there is some overlap with the normal range. There ...
متن کاملEvaluation of a radioimmunoassay for estradiol in unextracted serum.
We describe the performance of a commercial (Steranti/EIR) RIA reagent kit for measuring 17 beta-estradiol directly in serum. Day-to-day precision data for control sera were as follows: mean = 102.8 ng/L, CV = 6.8%, n = 20; mean = 231.1 ng/L, CV = 5.3%, n = 21; mean = 747.7 ng/L, CV = 9.4%, n = 21. Analytical recovery of added estradiol from seven different serum pools from men, to which three ...
متن کاملA direct radioimmunoassay for aldosterone in unextracted serum and plasma.
We describe a novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme. Heating at 75 degrees C for 15 min inactivates the enzyme before radioimmunoassay. Alternatively, EDTA may be used to in...
متن کاملSolid-phase chemiluminescence immunoassay for progesterone in unextracted serum.
We describe a simple, solid-phase chemiluminescence immunoassay for progesterone in 10 microL of unextracted serum ("direct" assay). Danazol at pH 8.0 is included (100 ng per tube) to displace progesterone from binding proteins in serum. A progesterone-11 alpha-hemisuccinyl-aminobutylethyl isoluminol conjugate serves as the chemiluminescent ligand marker and homologous antiprogesterone IgG cova...
متن کاملRadioimmunoassay of thyroxine in unextracted serum, by a single-antibody technique.
A radioimmunoassay procedure for thyroxine (14) is described, for which only 25 il of serum is required. The antibody, commercially available, is produced in rabbits in response to injections of a T4-albumin conjugate. Native T4-binding proteins are denatured by heating 25 .tl of serum in 0.2 ml of a glutamate buffer in a boiling water bath. After a 1-h incubation of heat-treated serum with l12...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1980
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/26.11.1607